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To give your Twitch VOD a different audio mix from your live stream in Streamlabs Desktop, enable Twitch VOD under Settings > Output, then choose which audio sources feed the VOD track in Advanced Audio Options. The default VOD track is Track 2. This controls Twitch’s VOD audio; it is separate from local multi-track recording.
Set up the Twitch VOD track
- Make Twitch the destination. Select Twitch as your streaming platform. If you use Dual Output, make sure one destination is Twitch. Streamlabs says the Twitch VOD feature is not currently available for multistream. See the Streamlabs Twitch VOD Track guide, last updated March 17, 2026.
- Open the output settings. Go to Settings > Output.
- Enable the feature for your output mode.
- In Simple output mode, turn on Advanced encoder settings, then enable Twitch VOD.
- In Advanced output mode, enable Twitch VOD directly.
- Choose the audio sources for the VOD. In the audio mixer, select the gear icon to open Advanced Audio Options. For each source, check the selected VOD track if you want that audio included in the Twitch VOD; uncheck it to exclude that source. Track 2 is the default VOD track, but you can change the selected track.
- Check the result on a VOD. The source assignments determine the VOD mix, separately from the live mix. After streaming, review the VOD to confirm the intended sources are present or absent.
What “separate Twitch VOD track” means
This feature routes selected audio sources to Twitch’s VOD output. For example, you can exclude a music source from the VOD while keeping it in the live mix. It does not create editable audio stems in a local file. Streamlabs describes the feature and its source assignments in its Twitch VOD setup guide.
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When you need separate audio in a local recording
Local multi-track recording is a different workflow: it saves audio inputs on separate tracks in a recording file so they can be handled separately in an editor. Streamlabs documents configuring this under Output > Recording in Advanced output mode, then assigning sources in Advanced Audio Settings. Its documented workflow supports up to six audio tracks. The Streamlabs Desktop recording guide was last updated September 22, 2026.
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Configure a local multi-track recording
- Open Settings > Output and switch the output mode to Advanced.
- Open the Recording section and choose the number of audio tracks to record, up to six.
- Open Advanced Audio Settings and assign each audio input to the track or tracks you want it recorded on.
- Choose a recording format that supports multiple audio tracks. Streamlabs says FLV does not support multiple audio tracks and advises MP4 or MKV.
- Record a short test and check the file in your editing software to confirm the tracks are available as intended.
These recording assignments affect the local file, not what Twitch saves in its VOD. For Streamlabs’ multi-track instructions, see How to use multi-track recording in Streamlabs Desktop, last updated April 25, 2025.
#1 Best Overall
Focusrite Scarlett Solo 3rd Gen USB-C Audio Interface | 1 XLR Mic Preamp, 1 Hi-Z Instrument Input, 24-Bit/192kHz, 56dB Gain Range, Air Mode, macOS/Windows/iPadOS Compatible
- Pro performance with great pre-amps - Achieve a brighter recording thanks to the high performing mic pre-amps of the Scarlett 3rd Gen. A switchable Air mode will add extra clarity to your acoustic instruments when recording with your Solo 3rd Gen
- Get the perfect guitar and vocal take with - With two high-headroom instrument inputs to plug in your guitar or bass so that they shine through. Capture your voice and instruments without any unwanted clipping or distortion thanks to our Gain Halos
- Studio quality recording for your music & podcasts - Achieve pro sounding recordings with Scarlett 3rd Gen’s high-performance converters enabling you to record and mix at up to 24-bit/192kHz. Your recordings will retain all of their sonic qualities
- Low-noise for crystal clear listening - 2 low-noise balanced outputs provide clean audio playback with 3rd Gen. Hear all the nuances of your tracks or music from Spotify, Apple & Amazon Music. Plug-in headphones for private listening in high-fidelity
- Everything in the box: Includes Pro Tools Intro+, Ableton Live Lite, Cubase LE, and Hitmaker Expansion: a suite of essential effects, powerful software instruments, and easy-to-use mastering tools
Troubleshoot a VOD that has the wrong audio
- The Twitch VOD option is missing: confirm Twitch is the selected destination and check whether you are multistreaming. Streamlabs says Twitch VOD is not currently available for multistream. In Simple mode, enable Advanced encoder settings to expose the option.
- A source is audible in the VOD when it should be excluded: reopen Advanced Audio Options from the mixer gear and uncheck that source’s selected VOD track.
- A wanted source is missing from the VOD: check that source’s VOD-track assignment. The VOD track defaults to Track 2, so verify the selected track matches the one enabled for Twitch VOD.
- You expected separate tracks in your recording file: Twitch VOD routing does not create local stems. Configure local multi-track recording under Output > Recording instead, and use MP4 or MKV rather than FLV.
Or let it run in the cloud
StreamNeo is for a different job: keeping an uploaded video or playlist live on a YouTube channel 24/7. It does not configure Twitch VOD audio, stream from a camera, or send a stream to Twitch.
- Upload a recording or build a playlist.
- Add your YouTube stream key once.
- Go live; StreamNeo loops the video from the cloud.
Your computer and home connection do not have to stay on. Each slot streams your upload as made, up to 4K 60fps, at one flat price per slot; there are no quality tiers or re-encoding. StreamNeo automatically recovers if YouTube drops the stream. The first day is free with no card, one free day per account. Monthly is $9.99 per month. Learn more at StreamNeo, or start your free day.
Quick Recap
Bestseller No. 4
Best Value
Focusrite Scarlett 2i2 4th Gen USB-C Audio Interface | 2 Mic Preamps, 2 Hi-Z Instrument Inputs, 24-Bit/192kHz, 120dB Dynamic Range, Auto Gain, Clip Safe, Air Modes
- The new generation of the artist's interface: Connect your mic to Scarlett's 4th Gen mic pres. Plug in your guitar. Fire up the included software. Start making your first big hit
- Studio-quality sound: With a huge 120dB dynamic range, the newest generation of Scarlett uses the same converters as Focusrite’s flagship interfaces, found in the world's biggest studios
- Never lose a great take: Scarlett 4th Gen's Auto Gain sets the perfect level for your mic or guitar, and Clip Safe prevents clipping, so you can focus on the music
- Find your signature sound: Air mode lifts vocals and guitars to the front of the mix, adding musical presence and rich harmonic drive to your recordings
- With Scarlett 4th Gen, you have all you need to record, mix and master your music: Includes industry-leading recording software and a full collection of record-making plugins
Rank #4
M-AUDIO M-Track Duo USB Audio Interface | Dual XLR, Line and DI Inputs, Professional Software Suite for Recording, Streaming, and Podcasting
- Podcast, Record, Live Stream, This Portable Audio Interface Covers it All - USB sound card for Mac or PC delivers 48kHz audio resolution for pristine recording every time
- Be ready for anything with this versatile M-AUDIO interface - Record guitar, vocals or line input signals with two combo XLR / Line / Instrument Inputs with phantom power
- Everything you Demand from an Audio Interface for Fuss-Free Monitoring - 1/4" headphone output and stereo 1/4" outputs for total monitoring flexibility; USB/Direct switch for zero latency monitoring
- Get the best out of your Microphones - M-Track Duo’s transparent Crystal Preamps guarantee optimal sound from all your microphones including condenser mics
- The MPC Production Experience - Includes MPC Beats Software complete with the essential production tools from Akai Professional
Rank #3
SABRENT USB External Stereo Sound Card Adapter, Plug & Play (AU-MMSA) | USB audio adapter; no drivers needed; for Windows & Mac; 3.5mm headphone & mic jacks; usb to 3.5mm jack audio adapter
- PLUG IN AND HEAR SOUND IN SECONDS - USB Type-A connector with a 3.5mm stereo headphone output and a separate 3.5mm mono microphone input. No drivers, no software, no external power - the adapter is USB bus-powered and is recognized as a standard USB audio device.
- WORKS ON WINDOWS, MAC AND LINUX - Driverless on Windows 98SE/ME/2000/XP/Server 2003/Vista/7/8, Linux and Mac OSX, and compliant with the USB Audio Device Class 1.0 specification, so any system that supports class-compliant USB audio will see it. Select it as the sound output and input device after plugging it in.
- TWO JACKS, TWO JOBS - The green jack is stereo OUT for headphones or powered speakers; the pink jack is mono microphone IN for a 3.5mm mic. It does NOT support 4-pole headsets on a single combo plug, it does NOT power passive speakers, and it does NOT add surround sound - it is a stereo 2-channel adapter.
- FOR LAPTOPS AND DESKTOPS THAT NEED AN AUDIO PORT BACK - Adds a headphone and mic port to a laptop, desktop, or mini PC whose onboard jack has failed or was never there. Managed and work-issued computers can block new USB audio devices by policy - check with your IT department before ordering for a company machine.
- SABRENT SUPPORT AND WARRANTY - What is in the box: one USB audio sound adapter. Backed by a 1-year limited warranty, extended to 2 years when you register within 90 days on the manufacturer's website.
Rank #2
Focusrite Scarlett Solo 4th Gen USB-C Audio Interface | 1 XLR Mic Preamp, 1 Hi-Z Instrument Input, 24-Bit/192kHz, 120dB Dynamic Range, Air Modes, PC/Mac/iPad Compatible
- The new generation of the songwriter's interface: Plug in your mic and guitar and let Scarlett Solo 4th Gen bring big studio sound to wherever you make music
- Studio-quality sound: With a huge 120dB dynamic range, the newest generation of Scarlett uses the same converters as Focusrite’s flagship interfaces, found in the world's biggest studios
- Find your signature sound: Scarlett 4th Gen's improved Air mode lifts vocals and guitars to the front of the mix, adding musical presence and rich harmonic drive to your recordings
- All you need to record, mix and master your music: Includes industry-leading recording software and a full collection of record-making plugins
- Everything in the box: Includes Pro Tools Intro+, Ableton Live Lite, Cubase LE, and Hitmaker Expansion: a suite of essential effects, powerful software instruments, and easy-to-use mastering tools
Product prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on Amazon at the time of purchase will apply.
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