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Clear out junk files and repair common Windows errorsFree Scan →Fix the driver behind crashes, sound loss and screen glitchesFind Drivers →Repair Windows errors before they cause bigger problemsFix Now →A project called FlyCNS-TicTacToe routes a tic-tac-toe board through a selected circuit from the male fruit fly connectome. It is a programming experiment, not a fly playing a game: the board encoding and move-selection rules are engineered, and the default minimax setting protects the game-theoretic result while the circuit breaks ties.
What the project connects
Rishu’s FlyCNS-TicTacToe project uses a subset of the male Drosophila central nervous system connectome. Its documented dataset is male-cns:v1.0, hosted through neuPrint. The extraction pipeline selects descending neurons and upstream central-brain intrinsic neurons, ranks candidate partners by synaptic weight, groups outputs into nine readouts corresponding to board cells, and retains the largest connected component of the selected circuit.
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The source wiring, neuron identities, synapse counts, and predicted neurotransmitter metadata come from connectome data. The mapping from those neurons to a nine-cell game board, and the interpretation of circuit outputs as moves, are project design choices.
How a board becomes a circuit input
The game layer divides selected central-brain neurons into nine input groups, one for each square. A mark from one player injects a positive signal into its group; the opponent’s mark injects a negative signal. Empty squares contribute no signal.
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Signals propagate across signed connectome edges using a simplified leaky-integrator calculation. The circuit’s strongest empty output group is treated as its preferred move. The project names neuprint-python, networkx, and pandas among its tools.
Edge signs are inferred from database-predicted neurotransmitter identities: acetylcholine is mapped to excitatory, GABA and glutamate to inhibitory, and other predictions to neutral. These transmitter assignments are predictions, not certain measurements, so the signs are part of the model rather than definitive labels for every biological connection.
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Does the fly circuit actually choose the move?
In the default configuration, unbeatable=True, exact minimax first identifies the game-theoretically optimal legal moves. The connectome then chooses among that set. Minimax determines whether the protected player can force a win, draw, or loss from the current position; the circuit acts as a tie-breaker and may have no meaningful choice if only one optimal move exists.
With unbeatable=False, the safety filter is removed and the circuit’s preferred move can be suboptimal. The project author notes that the raw circuit can lose. The available project materials do not report a win rate or other performance benchmark.
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The opening is deterministic: an empty board produces a flat zero activation, and a tie is broken using the output group’s input weight. The author says identical boards yield identical moves, but a likely square bias has not been measured. That should not be mistaken for evidence of a strategically meaningful opening preference.
What this experiment does—and does not—model
No real fly sees a tic-tac-toe grid, and the selected subgraph does not include a biological sensory pathway that supplies board states. The board-to-neuron inputs are supplied by the program.
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The simulation is illustrative signal flow, not a biophysical fly-brain model. It uses leaky-integrator dynamics and does not model spikes, real time constants, refractory periods, or real biological timing. The biological data supplies a connectivity-derived circuit; the game, its encoding, and the simplified simulation are engineering choices.
How to run the project
The repository’s documented workflow uses uv and neuPrint access. A free neuPrint token is required according to the project author. Service access details and repository instructions can change, so consult the project repository and neuPrint for current requirements.
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- Clone the repository and install its dependencies with
uv sync. - Configure a neuPrint token as described in the repository.
- Set the neuPrint host to
neuprint-cns.janelia.organd select datasetmale-cns:v1.0. - Run the repository’s extraction pipeline to build the selected circuit.
- Start the terminal game using the command documented in the repository.
Code and connectome data have separate licenses
The repository identifies its code as MIT licensed and the connectome data as CC-BY licensed. These are distinct permissions: the code license does not replace the data license. If publishing outputs derived from the connectome, follow the repository’s direction to credit the dataset creators separately.
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