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VplotR

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What VplotR is

VplotR is a free desktop R package for analyzing paired-end genomic sequencing data as two-dimensional fragment-density V-plots. It imports BAM files from DNA-accessibility experiments such as ATAC-seq, DNA-seq, and MNase-seq. The plotVmat() function calculates fragment density over selected genomic loci, while plotFootprint() creates MNase-seq or ATAC-seq footprint profiles across genomic ranges. Other functions examine fragment-size distributions and paired-end fragment profiles at individual loci. Built around ggplot2, VplotR lets users combine its graphics with other plots for publication-ready figures. V-plots can use raw counts, library-depth or locus-count normalization, z-scores, and quantile scaling. Multiple plots can run in parallel using a parameter list and cores setting. Example data include C. elegans ATAC-seq, yeast MNase-seq, and a sample BAM file. The package integrates with Bioconductor, requires R 4.0 or later, and is licensed under GPL-3.

Who it suits

VplotR is suited to researchers working with paired-end accessibility sequencing data. It also fits users who need ggplot2-compatible figures, footprint profiles, normalization choices, or parallel plot generation.

What it does well

  • Imports ATAC-seq, DNA-seq, and MNase-seq BAM files
  • Creates V-plots and footprint profiles
  • Supports several normalization methods
  • Works with ggplot2 and Bioconductor
  • Includes example sequencing datasets

What to know first

  • Requires R version 4.0 or later
  • It is a desktop R package, not a hosted service
  • Parallel plotting requires configuring parameters and cores

Plans

PlanBilled and limitsPrice
VplotRGPL-3 licensed R package · Requires R >= 4.0 · Available from Bioconductor release > 3.12Free

As jserizay.com lists them, read 12 Oct 2026. The maker's own page has the final word.

Verdict

VplotR focuses on specialized visualization and fragment analysis for DNA-accessibility sequencing. Its Bioconductor and ggplot2 integration, example data, and normalization options support research workflows, provided the R requirement is met.

Facts from VplotR's own pages

Bioconductor integration
The package is integrated with the Bioconductor environment and can be installed from Bioconductor. · jserizay.com, 12 Oct 2026
Data sources
Genomic sequencing data · jserizay.com, 24 Sept 2026
Footprints
The plotFootprint() function generates MNase-seq or ATAC-seq footprint profiles across genomic ranges. · jserizay.com, 12 Oct 2026
Fragment analyses
The package includes functions for fragment-size distributions and paired-end fragment profiles at individual loci. · jserizay.com, 12 Oct 2026
Free plan
Yes · jserizay.com, 24 Sept 2026
Included datasets
The package includes example ATAC-seq datasets from C. elegans, a yeast MNase-seq dataset, and a sample BAM file. · jserizay.com, 12 Oct 2026
License
VplotR is licensed under GPL-3. · jserizay.com, 12 Oct 2026
Maintainer
Jacques Serizay is listed as the package author and maintainer. · jserizay.com, 12 Oct 2026
Normalization
V-plots support raw counts, library-depth and locus-count normalization, z-scores, and quantile scaling. · jserizay.com, 12 Oct 2026
Parallel plotting
Generation of multiple V-plots can be parallelized using a list of parameters and a cores setting. · jserizay.com, 12 Oct 2026
Platform
desktop · jserizay.com, 24 Sept 2026
Plotting integration
VplotR is designed around ggplot2 so plots can be combined with other plots to create publication-ready figures. · jserizay.com, 12 Oct 2026
Purpose
VplotR is an R package for generating two-dimensional paired-end fragment density plots called V-plots. · jserizay.com, 12 Oct 2026
Requirements
VplotR requires R >= 4.0 and has been tested on Mac OS >= 10.13, Ubuntu >= 18.04, and Windows Server 2012. · jserizay.com, 12 Oct 2026
Support
The project invites bug reports and feature requests through GitHub issues and lists an email address for queries to J. Serizay. · jserizay.com, 12 Oct 2026
Supported assays
It imports paired-end sequencing BAM files from DNA accessibility experiments including ATAC-seq, DNA-seq, and MNase-seq. · jserizay.com, 12 Oct 2026
V-plot generation
The plotVmat() function computes fragment density and generates V-plots over genomic loci of interest. · jserizay.com, 12 Oct 2026

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