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How to Perform Hypothesis Testing in Python

A practical guide to hypothesis testing in Python: define hypotheses, choose a test for your data and design, run Welch’s t-test in SciPy, and report results carefully.
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To perform hypothesis testing in Python, define the null and alternative hypotheses, match a statistical test to your outcome and study design, check its assumptions, then interpret the test statistic and p-value alongside an effect estimate and uncertainty interval. For two independent groups with a numeric outcome, SciPy’s ttest_ind is a common choice; set equal_var=False to use Welch’s t-test when you do not want to assume equal population variances.

1. Define the question before choosing a test

State the population quantity or relationship you want to learn about. Then specify:

  • Null hypothesis (H₀): the reference claim, such as equal population means.
  • Alternative hypothesis (H₁): the difference or relationship you want to detect. Choose a two-sided or directional alternative before examining the result.
  • Unit of observation: the person, transaction, device, or other unit that contributes an observation. Measurements from the same unit are not independent merely because they occupy separate rows.

Also identify the outcome: numeric measurements, binary outcomes, or counts in categories call for different methods. A test’s name or a familiar Python function does not establish that it fits your question.

2. Choose a test that matches the data and design

Use the outcome type, number of samples, pairing, target quantity, assumptions, and desired reporting information to narrow the options. SciPy groups its procedures by common use and documents their differing assumptions in its statistical reference; its hypothesis-testing tutorial includes chi-square and Fisher exact examples.

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Question or design Possible direction Important distinction
Compare means for two independent numeric samples Independent-samples t-test, such as SciPy’s ttest_ind Its default assumes equal population variances; equal_var=False requests Welch’s test.
Compare measurements from the same units or matched pairs A paired procedure Do not treat paired or repeated measurements as independent samples.
Analyze categorical counts or association in a contingency table A suitable chi-square or Fisher exact procedure Choose based on the table and whether the test’s approximation is suitable.
Test a proportion or calculate a proportion interval Statsmodels functions such as proportions_ztest or proportion_confint These answer proportion questions, not mean-comparison questions.

Statsmodels documents proportion tests, confidence intervals, and related procedures in its statistics reference. These methods are alternatives for different questions and data conditions, not interchangeable fixes for a poorly specified design.

3. Run a two-independent-group test in SciPy

For two independent numeric samples, this example performs Welch’s two-sided t-test and prints the statistic, degrees of freedom, p-value, and a 95% confidence interval for the difference in population means:

from scipy import stats

# group_a and group_b should contain independent observations
# of the same numeric outcome.
result = stats.ttest_ind(
    group_a,
    group_b,
    equal_var=False,          # Welch's t-test
    alternative="two-sided",
    nan_policy="omit",
)

print(f"t = {result.statistic:.3f}")
print(f"df = {result.df:.1f}")
print(f"p = {result.pvalue:.4g}")
print(result.confidence_interval(confidence_level=0.95))

Consult the official SciPy ttest_ind reference for the function’s arguments and returned result. The function supports alternative='two-sided', 'less', and 'greater'. Its default equal_var=True requests the conventional pooled-variance independent t-test; choose equal_var=False when using Welch’s procedure. The result provides a statistic, p-value, degrees of freedom, and a confidence_interval() method for the difference in population means.

Handle missing values deliberately

nan_policy='omit' excludes missing observations from the calculation. Use it only if dropping those observations is substantively appropriate. Consider why values are missing and whether their absence could bias the comparison; silent omission is not a missing-data analysis.

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Check pairing and independence

The example is specifically for independent samples. If both measurements come from the same people, devices, or matched units, use a paired method. If observations are clustered or otherwise dependent, account for that design rather than treating every row as an independent observation.

4. Check assumptions and analysis choices

  • Design: confirm that the procedure matches independent, paired, or repeated observations.
  • Outcome and target: confirm that the test addresses the variable type and quantity in your question.
  • Variance handling: for the independent t-test, be explicit about the equal-variance assumption or use Welch’s option.
  • Missing data: decide how missing observations should be handled and explain the choice.
  • Alternative direction: set two-sided or directional testing before looking at the result; do not choose a direction afterward to obtain a smaller p-value.
  • Approximation: for categorical-count tests, check whether the chosen procedure’s approximation is appropriate for the data.

5. Interpret the result without overclaiming

A p-value is calculated under the null model. It describes the probability of observing results at least as extreme as those obtained if that model holds; it is not the probability that the null hypothesis is true. SciPy’s ttest_ind documentation describes it this way: “The p-value quantifies the probability of observing as or more extreme values assuming the null hypothesis, that the samples are drawn from populations with the same population means, is true.” See the official reference.

Choose a significance threshold as part of the analysis plan, not after seeing the p-value. If the p-value is below that threshold, describe evidence against the stated null under the selected model; do not say the null has been proven false. If it is above the threshold, say the analysis did not provide sufficient evidence to reject the null. That result does not prove equality or show that an effect is absent.

Statistical significance alone does not convey practical magnitude. Report an estimated difference and confidence interval where available, as well as group sizes and useful descriptive summaries. For the example above, the interval is for the difference in population means; make clear which group is subtracted from which when presenting the estimate.

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6. Common problems and fixes

  • Independent-samples test used for paired data: switch to a paired procedure that reflects the repeated or matched measurements.
  • Default variance assumption overlooked: ttest_ind defaults to equal_var=True. Set equal_var=False if using Welch’s test, and state the choice.
  • Missing values produce an unusable result: inspect the input and choose a missing-data approach deliberately. If omission is justified, set nan_policy='omit' and report the choice.
  • Directional alternative chosen after seeing the data: define the direction in advance. If the question is not directional, use a two-sided alternative.
  • Non-significant result reported as “no effect”: report that the test did not provide sufficient evidence to reject the null, then show the estimate and interval so readers can assess plausible magnitudes.
  • Test selected from a function name alone: revisit the outcome type, target quantity, independence, pairing, and assumptions; use the official SciPy test reference to compare procedures.

7. Skip screenshot setup for Python documentation or results

Hypothesis testing itself does not require a screenshot service. If a workflow also needs to capture a page—such as a rendered report or documentation URL—ScreenshotNeo is a website screenshot API and MCP server for developers. It can return an image or PDF from one GET request.

Or skip the browser setup:

curl -G "https://api.screenshotneo.com/v1/shot" -d access_key=YOUR_API_KEY --data-urlencode url=https://stripe.com -o shot.webp

See the ScreenshotNeo API documentation for options. It accepts cookie and consent banners before capture and removes more than 60 known consent platforms, newsletter popups, and chat widgets; each step can be turned off. Bot checks and CAPTCHAs, blank pages, timeouts, failed loads, and cache hits are not billed, with response headers indicating the page verdict and billing. An MCP server provides take_screenshot, get_page_info, and capture_pdf for AI agents and other MCP clients. The free plan includes 1,000 shots per month with no card; paid plans start at $5 for 3,000 shots.

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